l paracasei subsp paracasei atcc 25302  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Lactobacillus paracasei WIS43 alleviates DSS-induced colitis by modulating gut microbiota and suppressing inflammation

doi: 10.3389/fmicb.2025.1721585

Figure Lengend Snippet: Effects of Lactobacillus paracasei interventions on gut microbiota composition in DSS-induced colitis mice. (A) Alpha diversity indices (Chao1, Shannon, Simpson). WIS43 significantly increased Shannon and Simpson indices compared with DSS, indicating partial restoration of microbial richness and evenness. (B) Principal coordinate analysis (PCoA) based on Bray–Curtis distances revealed distinct clustering of microbial communities among groups (PERMANOVA, R 2 = 0.33, p = 0.001). (C) Non-metric multidimensional scaling (NMDS) analysis confirmed separation of microbial compositions, with a stress value of 0.176, indicating reliable ordination. For microbiota analysis, n = 6 samples were randomly selected from each group. (D,E) Relative abundance of gut microbiota at the genus (D) and species (E) levels. WIS43 restored the abundance of beneficial taxa such as L. johnsonii and L. animalis while reducing potentially pathogenic taxa including Kineothrix and Duncaniella .

Article Snippet: The neighbor-joining tree demonstrated that WIS43 clustered robustly with L. paracasei lineages, forming a well-supported clade with L. paracasei subsp. paracasei ATCC 25302 and L. paracasei subsp. tolerans JCM 1171, with bootstrap values exceeding 90% ( ).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Comprehensive Safety Assessment of Lacticaseibacillus paracasei subsp. paracasei NTU 101 Through Integrated Genotypic and Phenotypic Analysis

doi: 10.3390/cimb46110734

Figure Lengend Snippet: Antibiotic resistance results of NTU 101 through genotypic and phenotypic analysis. Antimicrobial minimum inhibitory concentrations (MICs) results compare to L. paracasei subsp. paracasei BCRC 12248 T .

Article Snippet: Furthermore, the TYGS analysis indicated that both the phylogenetic tree based on 16S rDNA sequences and the phylogenomic tree based on whole genome sequences showed that NTU 101 is more closely related to the L. paracasei subsp. paracasei type strains (JCM 8130 T and ATCC 25302 T ) ( ).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Comprehensive Safety Assessment of Lacticaseibacillus paracasei subsp. paracasei NTU 101 Through Integrated Genotypic and Phenotypic Analysis

doi: 10.3390/cimb46110734

Figure Lengend Snippet: Genotypic analysis of the hemolysis-related genes in NTU 101.

Article Snippet: Furthermore, the TYGS analysis indicated that both the phylogenetic tree based on 16S rDNA sequences and the phylogenomic tree based on whole genome sequences showed that NTU 101 is more closely related to the L. paracasei subsp. paracasei type strains (JCM 8130 T and ATCC 25302 T ) ( ).

Techniques:

Journal: Current Issues in Molecular Biology

Article Title: Comprehensive Safety Assessment of Lacticaseibacillus paracasei subsp. paracasei NTU 101 Through Integrated Genotypic and Phenotypic Analysis

doi: 10.3390/cimb46110734

Figure Lengend Snippet: The results of the mucin degradation activity assay of NTU 101 and reference strains. PSM is the medium with mucin as the sole carbon source. PSMG is the PSM with additional glucose. Each number represents the following strain: (1) L. paracasei subsp. paracasei NTU 101. (2) L. paracasei subsp. paracasei BCRC 17002. (3) L. paracasei subsp. paracasei BCRC 12248 T . (4) L. paracasei subsp. paracasei BCRC 14023. (5) L. rhamnosus BCRC 16000. (6) S. enterica subsp. enterica BCRC 10747 (mucin degradation activity-positive strain).

Article Snippet: Furthermore, the TYGS analysis indicated that both the phylogenetic tree based on 16S rDNA sequences and the phylogenomic tree based on whole genome sequences showed that NTU 101 is more closely related to the L. paracasei subsp. paracasei type strains (JCM 8130 T and ATCC 25302 T ) ( ).

Techniques: Activity Assay

Journal: Current Issues in Molecular Biology

Article Title: Comprehensive Safety Assessment of Lacticaseibacillus paracasei subsp. paracasei NTU 101 Through Integrated Genotypic and Phenotypic Analysis

doi: 10.3390/cimb46110734

Figure Lengend Snippet: Results of mucin degradation ability of each strain, Salmonella enterica subsp. enterica BCRC 10747 as a positive strain.

Article Snippet: Furthermore, the TYGS analysis indicated that both the phylogenetic tree based on 16S rDNA sequences and the phylogenomic tree based on whole genome sequences showed that NTU 101 is more closely related to the L. paracasei subsp. paracasei type strains (JCM 8130 T and ATCC 25302 T ) ( ).

Techniques:

Journal: Nature Communications

Article Title: A lactobacilli-based inhaled live biotherapeutic product attenuates pulmonary neutrophilic inflammation

doi: 10.1038/s41467-024-51169-0

Figure Lengend Snippet: A Individual strains L. plantarum (P), L. acidophilus (A), and L. rhamnosus (R) reduce MMP-9 expression more than individual strains L. casei, L. paracasei, L. reuteri , and L. fermentum compared to E. coli exposure alone with no Lactobacillus treatment and compared to each other. N = 7. B Different ratios of blended P, A, and R reduce MMP-9 to varying degrees. N = 7 wells. C A blend of P, A, and R performed better than individual strains in reducing MMP-9 in human bronchial epithelial (HBE) in vitro model of noxious stimuli exposure. N = 4 wells. D Lactobacillus blend culture supernatant in increasing concentrations reduces MMP-9 expression. N = 6. E Individual live Lactobacillus strains were transfected with supernatant from the other three strains in the blend. L. acidophilus supernatant cultured with live A, P, R, and PR blend increased lactate gene expression of P and the PR blend (shown as a representative example). AS A N = 13, AS P N = 6, AS R N = 9, AS PR N = 9 wells. F Lactobacillus strains produce more L (+) lactic acid than D (+) lactic acid individually and as a blend. N = 6 wells. G L (+) lactic acid reduces MMP-9 expression in HBE cells exposed to E. coli ( N = 6 wells), while H D (−) lactic acid does not ( N = 4 wells). Kruskal–Wallis test, Dunn’s multiple comparisons. Cell culture performed in triplicate. Bars represent the median ± interquartile range. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: P, A, and R activity in reducing MMP-9 was tested against four additional strains: L. casei (ATCC 393) , L. paracasei (ATCC 25302) , L. reuteri (ATCC 23272), and L. fermentum (ATCC 23271) .

Techniques: Expressing, In Vitro, Transfection, Cell Culture, Gene Expression